Control of benzylamine oxidase activity in Kluyveromyces fragilis grown on primary amines as nitrogen source

Transformation of Kluyveromyces fragilis.

For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment by alkali cations and with the Kmr gene as the selective marker, a wild-type s...

Production and purification of lactase of Kluyveromyces fragilis from whey

Primary consumer stable nitrogen isotopes as indicators of nutrient source.

Non-point source loading of nitrogen and phosphorus is a primary cause of eutrophication of inland waters, although the diffuse and variable nature of nutrient inputs makes it difficult to trace and identify nutrient pathways. Stable nitrogen isotope values (delta15N) in aquatic biota are thought to reflect anthropogenic nutrient inputs, and they may be a promising tool fortracing nutrient sour...

production and purification of lactase of kluyveromyces fragilis from whey

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Transfer of DNA killer plasmids from Kluyveromyces lactis to Kluyveromyces fragilis and Candida pseudotropicalis.

Killer plasmids pGKL1 and pGKL2 of double-stranded linear DNAs were transferred from Kluyveromyces lactis to strains of Kluyveromyces fragilis and Candida pseudotropicalis. The resultant killer strains produced 17-fold and 6-fold larger amounts of killer toxin than K. lactis did, respectively. The killer toxin produced by each species appeared to be a glycoprotein.